- Published on
Design of highly functional genome editors by modelling CRISPR–Cas sequences
- Authors
- Name
- Vishal V
- @VishalVignesh_
Notes
CRISPR-based gene editors derived from microorganisms, although powerful, often show notable functional tradeoffs when ported into non-native environments, such as human cells (1)
we demonstrate successful precision editing of the human genome with a programmable gene editor designed with artificial intelligence (1)
1 million CRISPR operons (1)
Operon: genetic module containing multiple genes.
26 terabases of assembled genomes and metagenomes (1)
Terabase: 1 Trillion Basepairs
26 trillion * 2 bits = 52 trillion bits
52 trillion bits / 8 bits per byte = 6.5 terabyte
A, T, G, C are bases
A-T is a basepair G-C is a basepair
4.8× the number of protein clusters across CRISPR–Cas families found in nature and tailoring single-guide RNA sequences (1)
sgRNA is an engineered RNA molecule that directs Cas9 protein to a specific DNA location.
sgRNA has 2 parts:
Guide Sequence: Recognize target DNA, through complementary base pairing
Scaffold: Folds into a 3D structure to help Cas9 do its function on DNA
improved activity and specificity relative to SpCas9, the prototypical gene editing effector, while being 400 mutations away in sequence (1)
SpCas9: Widely used Cas9 protein from Streptococcus pyogenes.
SpCas9 contains 1368 amino acids.
A difference of:
400/1568 * 100 = 29%
OpenCRISPR-1, exhibits compatibility with base editing (1)